The origin of the Horned Serpent symbol derives from the ancient Mississippian culture of North America, the culture of the Mound Builders. The Mound Builders associated great mystical value to the serpent. Some Indian tribes  still retain some elements of the Mississippi culture. The Avanyu and the the horned serpent both feature in Hopi and other Pueblo art. The Navajo creation story features myths relating to twins and a horned monster. Their sacred rites, myths and symbols and are presumed to descend from the Mississippians. The Horned Serpent symbol was usually viewed as a benign or benevolent although fearful creature whereas the Great Serpent was usually represented as a malevolent creature.

Horned Serpent Rock Picture

The Meaning of the Horned Serpent Symbol
The Horned Serpent symbol is one of the many snake-like deities depicted with horns that figure in the mythology of most Native American tribes. The horned serpent is associated with rain, thunder and waterways. The zigzag and meandering lines of the serpent or snake are symbolic of water. The horned serpent symbol meaning represented life and the renewal of life, just like water. The horned serpent was the feared guardian of life and the forces of life. The Choctaw horned serpent deity called 'Sint holo' is seen as a source of inspiration. An inventive and creative spirit who introduced agriculture, language and other gifts of knowledge to man. The Choctaw tribe honored a spirit called 'Sinti lapitta' who was a horned serpent which visited exceptionally wise young men. Algonquin tribes belived in Mishikinebik, a great horned snake that was the guardian spirit brings that brings wisdom and healing. The Avanyu is probably the most famous horned serpent deity. Antlers and horns signified spiritual power, especially when applied to animals that did not ordinarily have them such as Birds, Panthers and Snakes (Serpents). Please refer to the Eye in the Hand Symbol for additional information.

The Horned Serpent Symbol - Mythology
Abenaki Horned Serpent Myth: The Abenaki deity Wa-won-dee-a-megw meaning "Snail" was a snail spirit that could live in trees, land or water. The spirit was able to change its size and appearance to look like a huge snake or serpent whose horns could be ground into a magical powder.